MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

Strain modifications for Medium 271

2 distinct modifications were found.

Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of complete medium to 2.0.

Modified strains

Acidithiobacillus thiooxidans DSM 11478

Annotated modifications

10 g Elemental sulfur
2.0
FeSO4 x 7 H2O

Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of complete medium to 2.5.

Modified strains

Acidithiobacillus sp. DSM 24413

Annotated modifications

10 g Elemental sulfur
FeSO4 x 7 H2O
2.5
Modifications
Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of complete medium to 2.0. Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of complete medium to 2.5.