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26 .
ACID RHODOSPIRILLACEAE MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Rhodomicrobium vannielii DSM 2342
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
28 °C
Main sol. 26 #
ml
Equipment needed: Tungsten lamp
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.20 | g | 0.2 | - | |
| Disodium succinate | 1.00 | g | 1 | 6.171 | |
| Ammonium acetate | 0.50 | g | 0.5 | 6.487 | |
|
Fe(III) citrate
(0.1% in H2O) |
5.00 | ml | 0.005 | 0.02 | |
| KH2PO4 | 0.50 | g | 0.5 | 3.674 | |
| MgSO4 x 7 H2O | 0.40 | g | 0.4 | 1.623 | |
| NaCl | 0.40 | g | 0.4 | 6.845 | |
| NH4Cl | 0.40 | g | 0.4 | 7.478 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
|
Vitamin B12
(10 mg in 100 ml H2O) |
0.40 | ml | - | - | |
| Trace element solution SL-6 | 1.00 | ml | - | - | |
| L-Cysteine HCl | 0.30 | g | 0.3 | 1.903 | |
|
Resazurin
(0,1%) |
0.50 | ml | 0.005 | 0.022 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 5.7. | |||||
| 2 Boil the medium for a few minute. | |||||
| 3 Sparge medium with 100% N2 gas mixture to make it anoxic. | |||||
| 4 Fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. | |||||
| 5 Autoclave at 121 °C for 20 min. | |||||
| 6 Sterile syringes are used to inoculate and remove samples. | |||||
| 7 Incubate in the light using a tungsten lamp. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
| H3BO3 | 0.30 | g | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.7 |
| Equipment needed: | Autoclave, Tungsten lamp |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 7 |
Rhodomicrobium vannielii DSM 2342
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Disodium succinate | 1 | 6.171 |
| Ammonium acetate | 0.5 | 6.487 |
| KH2PO4 | 0.5 | 3.674 |
| NH4Cl | 0.4 | 7.478 |
| NaCl | 0.4 | 6.845 |
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