Name: RHODOPSEUDOMONAS SULFOVIRIDIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 109 #

Equipment needed: Tungsten lamp Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract	0.30	g	0.3	-
Disodium succinate	1.00	g	1	6.171
Ammonium acetate	0.50	g	0.5	6.487
Fe(III) citrate (0.1% in H₂O)	5.00	ml	0.005	0.02
KH₂PO₄	0.50	g	0.5	3.674
MgSO₄ x 7 H₂O	0.40	g	0.4	1.623
NaCl	0.40	g	0.4	6.845
NH₄Cl	0.40	g	0.4	7.478
CaCl₂ x 2 H₂O	0.05	g	0.05	0.34
Vitamin B₁₂ (10 mg in 100 ml H₂O)	0.40	ml	-	-
Trace element solution SL-6	1.00	ml	-	-
L-Cysteine HCl	0.30	g	0.3	1.903
Resazurin (0,1%)	0.50	ml	0.005	0.022
Distilled water	1000.00	ml	-	-
1 To medium add 0.1% sodium malate and 0.05% sodium thiosulfate (). Adjust pH to 6.8 and sterilize at 121°C for 15 min. The liquid medium is sterilized in rubber stoppered or screw-capped bottles under anaerobic conditions (Ref. 3365). After sterilization neutralized sterile sodium sulfide solution is added to a final concentration of 0.03% (see medium 27).
Sodium malate	0.10	%	1	5.616
Sodium Thiosulfate	0.05	%	0.5	3.162
2 Incubate in the light using a tungsten lamp.
3 Adjust pH to 6.8.

Trace element solution SL-6 #

1000

Solution occurs in 55 other media (first defined in Medium 27).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.30	g	0.3	4.851
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.01	g	0.01	0.059
NiCl₂ x 6 H₂O	0.02	g	0.02	0.154
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-

All strains for this medium:

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26	ACID RHODOSPIRILLACEAE MEDIUM
27	RHODOSPIRILLACEAE MEDIUM (modified)
109	RHODOPSEUDOMONAS SULFOVIRIDIS MEDIUM
P7	N27 RHODOSPIRILLACEAE MEDIUM (modified)