# 26: ACID RHODOSPIRILLACEAE MEDIUM


## Main sol. 26

0.2	g	Yeast extract	
1	g	Disodium succinate	
0.5	g	Ammonium acetate	
5	ml	Fe(III) citrate (0.1% in H2O)	
0.5	g	KH2PO4	
0.4	g	MgSO4 x 7 H2O	
0.4	g	NaCl	
0.4	g	NH4Cl	
0.05	g	CaCl2 x 2 H2O	
0.4	ml	Vitamin B12 (10 mg in 100 ml H2O)	
1	ml	**Trace element solution SL-6**	
0.3	g	L-Cysteine HCl	
0.5	ml	Resazurin (0,1%)	
1000	ml	Distilled water	

1. Adjust pH to 5.7.
2. Boil the medium for a few minute.
3. Sparge medium with 100% N2 gas mixture to make it anoxic.
4. Fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.
5. Autoclave at 121 °C for 20 min.
6. Sterile syringes are used to inoculate and remove samples.
7. Incubate in the light using a tungsten lamp.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water