243
243 .
SEAWATER YEAST PEPTONE AGAR
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Vibrio harveyi DSM 6904
This strain uses the standard medium without modifications.
Main sol. 243 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Yeast extract | 3 | g | 3 | - | |
| Agar | 12 | g | 12 | 35.679 | |
| Distilled water | 250 | ml | - | - | |
|
Sea water
(filtered, aged) |
750 | ml | - | - | |
| 1 Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3. | |||||
| 2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||
Solution occurs in 5
other media (first defined in Medium 243).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 28.13 | g | 28.13 | 481.348 |
| KCl | 0.77 | g | 0.77 | 10.329 |
| CaCl2 x 2 H2O | 1.60 | g | 1.6 | 10.883 |
| MgCl2 x 6 H2O | 4.80 | g | 4.8 | 23.61 |
| NaHCO3 | 0.11 | g | 0.11 | 1.309 |
| MgSO4 x 7 H2O | 3.50 | g | 3.5 | 14.2 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 08.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 5 |
Vibrio harveyi DSM 6904
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 12 | 35.679 |
| Peptone | 5 | - |
| Yeast extract | 3 | - |
| Distilled water | - | - |
| Sea water | - | - |
