DSMZ 232 . MRS MEDIUM WITH CYSTEINE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Pectinatus frisingensis DSM 6306

* Modification: Plus 0.5% lactate, plus 1 drop/10 ml of NBB concentrate (Döhler); anaerobic

The modifications for this strain were extracted automatically and have not been curated by experts, yet.

Growth conditions: 28 °C
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone
(tryptic digest)
10.00 g 10 -
Meat extract 10.00 g 10 -
Yeast extract 5.00 g 5 -
Glucose 20.00 g 20 111.012
Tween 80 1.00 g 1 0.764
K2HPO4 2.00 g 2 11.483
Na-acetate 5.00 g 5 60.951
(NH4)2 citrate 2.00 g 2 -
MgSO4 x 7 H2O 0.20 g 0.2 0.811
MnSO4 x H2O 0.05 g 0.05 0.296
Lactate* 5.00 g/l - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.2 - 6.5.
2 Add 0.05% cysteine-hydrochloride.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.2 - 6.5
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Associated strains

All strains for this medium: 31
Strains with modifications: 15
Pectinatus frisingensis DSM 6306

  5.0 g/l Lactate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Glucose 20 111.012
Casein peptone 10 -
Meat extract 10 -
Na-acetate 5 60.951
Lactate 5 56.136
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Cultivation metadata from 31 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 1 strain
pH 4 - 6 1 strain
pH 2 - 4 1 strain
phyla 2 31 strains
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