# 232: MRS MEDIUM WITH CYSTEINE


## Main sol. 232

10	g	Casein peptone (tryptic digest)	
10	g	Meat extract	
5	g	Yeast extract	
20	g	Glucose	
1	g	Tween 80	
2	g	K2HPO4	
5	g	Na-acetate	
2	g	(NH4)2 citrate	
0.2	g	MgSO4 x 7 H2O	
0.05	g	MnSO4 x H2O	
1000	ml	Distilled water	

1. Adjust pH to 6.2 - 6.5.
2. Add 0.05% cysteine-hydrochloride.