Name: SACCHAROLOBUS SOLFATARICUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 182 #

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract (OXOID)	1.00	g	1	-
Casamino acids (BD BBL)	1.00	g	1	-
KH₂PO₄	3.10	g	3.1	22.78
(NH₄)₂SO₄	2.50	g	2.5	18.919
MgSO₄ x 7 H₂O	0.20	g	0.2	0.811
CaCl₂ x 2 H₂O	0.25	g	0.25	1.701
Allen's trace element solution	10.00	ml	-	-
Distilled water	1000.00	ml	-	-
1 Dissolve all compounds except yeast extract and Casamino acids, adjust pH to 4.0 - 4.2 with 10 N H₂SO₄ at room temperature and autoclave. Prior to inoculation add yeast extract and Casamino acids from stock solutions sterilized by filtration.

Allen's trace element solution #

1000

Solution occurs in 14 other media (first defined in Medium 88).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MnCl₂ x 4 H₂O	180	mg	0.18	0.91
Na₂B₄O₇ x 10 H₂O	450	mg	0.45	-
ZnSO₄ x 7 H₂O	22	mg	0.022	0.077
CuCl₂ x 2 H₂O	5	mg	0.005	0.029
Na₂MoO₄ x 2 H₂O	3	mg	0.003	0.012
VOSO₄ x 2 H₂O	3	mg	0.003	0.015
CoSO₄ x 7 H₂O	1	mg	1.0e-3	0.006
Distilled water	1000	ml	-	-
1 Adjust pH of final solution to 2 with 1 N HCl.

All strains for this medium:	3
Strains with modifications:	1

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182	SACCHAROLOBUS SOLFATARICUS MEDIUM
a	SACCHAROLOBUS (MT-4) MEDIUM