Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Brain heart infusion | 9.260 | g | - | - | |
Trypticase soy broth | 7.500 | g | - | - | |
Yeast extract | 2.500 | g | - | - | |
NaNO3 | 0.426 | g | - | - | |
K2HPO4 | 1.250 | g | - | - | |
1 Adjust pH to 7.0. | |||||
2 Bring to boil, cool down under N2CO2. | |||||
Cysteine HCl | 0.250 | g | - | - | |
3 Fill under N2CO2 in hungate tubes (10 ml) | |||||
4 Autoclave at 121 °C for 15 min. | |||||
Haemin solution | 0.050 | ml | - | - | |
Vitamin K3 solution | 0.050 | ml | - | - | |
Fetal bovine serum | 0.500 | ml | - | - | |
5 pressurize with 1 bar H2 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Haemin | 50 | mg | 0.5 | 0.767 | |
NaOH
(1 N) |
1 | ml | - | - | |
Distilled water | 100 | ml | - | - | |
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin K3 | 5 | mg | 0.05 | - | |
Ethanol
(95%) |
1 | ml | 9.5 | 206.208 | |
Water | 100 | ml | - | - | |
1 Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle. |
Last modified: | 26.07.22 |
Source: | MediaDive: DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Filter |
Description:
Special medium for Mucispirillum schaedleri. |
|
Edit in Medium builder |
All strains for this medium: | 1 |