Name: Mucispirillum Spezial
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. 1790 #

ml

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Brain heart infusion	9.260	g	-	-
Trypticase soy broth	7.500	g	-	-
Yeast extract	2.500	g	-	-
NaNO₃	0.426	g	-	-
K₂HPO₄	1.250	g	-	-
1 Adjust pH to 7.0.
2 Bring to boil, cool down under N₂CO₂.
Cysteine HCl	0.250	g	-	-
3 Fill under N₂CO₂ in hungate tubes (10 ml)
4 Autoclave at 121 °C for 15 min.
Haemin solution	0.050	ml	-	-
Vitamin K3 solution	0.050	ml	-	-
Fetal bovine serum	0.500	ml	-	-
5 pressurize with 1 bar H₂

Haemin solution #

100

Solution occurs in 15 other media (first defined in Medium 78).

Equipment needed: Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Haemin	50	mg	0.5	0.767
NaOH (1 N)	1	ml	-	-
Distilled water	100	ml	-	-
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

100

Solution occurs in 7 other media (first defined in Medium ).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Vitamin K₃	5	mg	0.05	-
Ethanol (95%)	1	ml	9.5	206.208
Water	100	ml	-	-
1 Dissolve 5 mg vitamin K₃ in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.

Last modified:	26.07.22
Source:	MediaDive: DSMZ
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.0
Equipment needed:	Autoclave, Filter
Description: Special medium for Mucispirillum schaedleri.

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phyla	1	1 strain
temperature	mesophilic	1 strain