Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Brain heart infusion | 9.260 | g | - | - | |
| Trypticase soy broth | 7.500 | g | - | - | |
| Yeast extract | 2.500 | g | - | - | |
| NaNO3 | 0.426 | g | - | - | |
| K2HPO4 | 1.250 | g | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Bring to boil, cool down under N2CO2. | |||||
| Cysteine HCl | 0.250 | g | - | - | |
| 3 Fill under N2CO2 in hungate tubes (10 ml) | |||||
| 4 Autoclave at 121 °C for 15 min. | |||||
| Haemin solution | 0.050 | ml | - | - | |
| Vitamin K3 solution | 0.050 | ml | - | - | |
| Fetal bovine serum | 0.500 | ml | - | - | |
| 5 pressurize with 1 bar H2 | |||||
Media with this solution
| 1790 | Mucispirillum Spezial |