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DSMZ

DSMZ 1727 . Z45/4 Medium for Cyanobacteria

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Stigonema ocellatum DSM 106950

* Modification: Standing culture, light cycle: 16 hours illumination at 150-200 lux and 8 hours dark period, transfer interval of 8-12 weeks

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: more than 4 weeks 17 °C

Main sol. 1727 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4
(0.41 g/l stock solution) 		100.00 ml 0.041 0.301
K2HPO4
(4.35 g/l stock solution) 		3.90 ml 0.017 0.098
CaCl2 x 2 H2O
(3.6 g/l stock solution) 		3.60 ml 0.037 0.252
MgSO4 x 7 H2O
(7.5 g/l stock solution) 		3.33 ml 0.025 0.101
Fe-EDTA complex 10.00 ml - -
Micronutrient solution (after H. Gaffron) 0.06 ml - -
1 Make up to 1000 ml with MilliQ water.
2 Autoclave at 121 °C for 20 min.

Fe-EDTA complex #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 135 mg 0.27 0.999
Na2-EDTA x 2 H2O 186 mg 0.372 1.106
1 Combine in deionized or distilled water and make up to 500 ml.

Micronutrient solution (after H. Gaffron) #

100
Solution occurs in 1 other media (first defined in Medium 1727).
1 Add to 100 ml of deionized or distilled water:
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 310.0 mg 3.1 50.129
MnSO4 x H2O 223.0 mg 2.23 13.194
Na2WO4 x 2 H2O 3.3 mg 0.033 0.1
(NH4)6Mo7O24 x 4 H2O 8.8 mg 0.088 0.071
KBr 11.9 mg 0.119 1
KJ 8.3 mg 0.083 0.341
ZnSO4 x 7 H2O 28.7 mg 0.287 0.998
Cd(NO3)2 x 4 H2O 15.4 mg 0.154 0.499
Co(NO3)2 x 6 H2O 14.6 mg 0.146 0.502
CuSO4 x 5 H2O 12.5 mg 0.125 0.501
(NH4)2Ni(SO4)2 x 6 H2O 19.8 mg 0.198 0.501
Cr(NO3)3 x 7 H2O 3.7 mg 0.037 0.102
VOSO4 x 2 H2O 2.0 mg 0.02 0.1
AlK(SO4)2 x 12 H2O 47.4 mg 0.474 0.999

Metadata

Last modified: 12.08.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave
Description:

Reference: A. Zehnder, EAWAG, ETH Zuerich, Switzerland
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Associated strains

All strains for this medium: 1
Strains with modifications: 1
Stigonema ocellatum DSM 106950

  150-200 lux

Light

Intensity 150-200 lux lux

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 0.041 0.301
CaCl2 x 2 H2O 0.037 0.252
MgSO4 x 7 H2O 0.025 0.101
K2HPO4 0.017 0.098
Na2-EDTA x 2 H2O 0.004 0.011
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain