1727 .
Z45/4 Medium for Cyanobacteria
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* Medium for Stigonema ocellatum DSM 106950
Standing culture, light cycle: 16 hours illumination at 150-200 lux and 8 hours dark period, transfer interval of 8-12 weeks
Growth conditions: more than 4 weeks
17 °C
Equipment needed:
Autoclave
Final pH:
n.d.
Description:
Reference: A. Zehnder, EAWAG, ETH Zuerich, Switzerland
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4
(0.41 g/l stock solution) |
100.00 | ml | ||
|
|
K2HPO4
(4.35 g/l stock solution) |
3.90 | ml | ||
|
|
CaCl2 x 2 H2O
(3.6 g/l stock solution) |
3.60 | ml | ||
|
|
MgSO4 x 7 H2O
(7.5 g/l stock solution) |
3.33 | ml | ||
|
|
Fe-EDTA complex | 10.00 | ml | ||
|
|
Micronutrient solution (after H. Gaffron) | 0.06 | ml | ||
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
Fe-EDTA complex #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeCl3 x 6 H2O | 135 | mg | ||
|
|
Na2-EDTA x 2 H2O | 186 | mg | ||
| 1 Combine in deionized or distilled water and make up to 500 ml. | |||||
Micronutrient solution (after H. Gaffron) #
100
| 1 Add to 100 ml of deionized or distilled water: | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
H3BO3 | 310.0 | mg | ||
|
|
MnSO4 x H2O | 223.0 | mg | ||
|
|
Na2WO4 x 2 H2O | 3.3 | mg | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 8.8 | mg | ||
|
|
KBr | 11.9 | mg | ||
|
|
KJ | 8.3 | mg | ||
|
|
ZnSO4 x 7 H2O | 28.7 | mg | ||
|
|
Cd(NO3)2 x 4 H2O | 15.4 | mg | ||
|
|
Co(NO3)2 x 6 H2O | 14.6 | mg | ||
|
|
CuSO4 x 5 H2O | 12.5 | mg | ||
|
|
(NH4)2Ni(SO4)2 x 6 H2O | 19.8 | mg | ||
|
|
Cr(NO3)3 x 7 H2O | 3.7 | mg | ||
|
|
VOSO4 x 2 H2O | 2.0 | mg | ||
|
|
AlK(SO4)2 x 12 H2O | 47.4 | mg | ||