1727
1727 .
Z45/4 Medium for Cyanobacteria
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1727 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
KH2PO4
(0.41 g/l stock solution) |
100.00 | ml | 234 | 1719.51 | |
|
K2HPO4
(4.35 g/l stock solution) |
3.90 | ml | - | - | |
|
CaCl2 x 2 H2O
(3.6 g/l stock solution) |
10.30 | ml | - | - | |
|
MgSO4 x 7 H2O
(7.5 g/l stock solution) |
3.33 | ml | - | - | |
| Fe-EDTA complex | 10.00 | ml | - | - | |
| Micronutrient solution (after H. Gaffron) | 0.06 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| FeCl3 x 6 H2O | 135 | mg | 0.27 | 0.999 | |
| Na2-EDTA x 2 H2O | 186 | mg | 0.372 | 1.106 | |
| 1 Combine in MilliQ water and make up to 500 ml. | |||||
| 1 Add to 100 ml of MilliQ water: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| H3BO3 | 310.0 | mg | 3.1 | 50.129 | |
| MnSO4 x H2O | 223.0 | mg | 2.23 | 13.194 | |
| Na2WO4 x 2 H2O | 3.3 | mg | 0.033 | 0.1 | |
| (NH4)6Mo7O24 x 4 H2O | 8.8 | mg | 0.088 | 0.071 | |
| KBr | 11.9 | mg | 0.119 | 1 | |
| KJ | 8.3 | mg | 0.083 | 0.341 | |
| ZnSO4 x 7 H2O | 28.7 | mg | 0.287 | 0.998 | |
| Cd(NO3)2 x 4 H2O | 15.4 | mg | 0.154 | 0.499 | |
| Co(NO3)2 x 6 H2O | 14.6 | mg | 0.146 | 0.502 | |
| CuSO4 x 5 H2O | 12.5 | mg | 0.125 | 0.501 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 19.8 | mg | 0.198 | 0.501 | |
| Cr(NO3)3 x 7 H2O | 3.7 | mg | 0.037 | 0.102 | |
| VOSO4 x 2 H2O | 2.0 | mg | 0.02 | 0.1 | |
|
AlK(SO4)2 x 12 H2O
(Synonym: Al2(SO4)3K2SO4 x 24 H2O) |
47.4 | mg | 0.474 | 0.999 | |
Metadata
| Last modified: | 19.03.25 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
|
Description:
Reference: A. Zehnder, EAWAG, ETH Zuerich, Switzerland |
|
| Edit in Medium builder | |
Light
| 1 strain needs light for growth. Select a strain for more details. |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KH2PO4 | 234 | 1719.51 |
| Na2-EDTA x 2 H2O | 0.004 | 0.011 |
| FeCl3 x 6 H2O | 0.003 | 0.01 |
| H3BO3 | 1.9e-4 | 0.003 |
| MnSO4 x H2O | 1.3e-4 | 7.9e-4 |
| Show more | ||
