167
167 .
SPOROCYTOPHAGA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Sporocytophaga myxococcoides DSM 11118
* Modification: It is recommended to use the following modified medium for the reactivation of freeze-dried samples: Prepare agar plates of medium 167 by supplementing medium with 1.5% (w/v) agar. Place 4 pieces of sterile cellulose (not nitrocellulose!) filter paper on each agar plate. Suspend freeze-dried content of the inner vial of one ampoule with 0.5 ml of liquid medium 167 and inoculate each piece of the filter paper with 1 large drop of the cell suspension. Alternatively, the strain also grows with 0.1% glucose or 0.1% cellobiose as carbon source.
Growth conditions: 3-7 days
aerobic
25 °C
Main sol. 167 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaNO3 | 2.00 | g | 2 | 23.531 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| KCl | 0.50 | g | 0.5 | 6.707 | |
| FeSO4 x 7 H2O | 6.00 | mg | 0.006 | 0.022 | |
| KH2PO4 | 0.14 | g | 0.14 | 1.029 | |
| K2HPO4 | 1.20 | g | 1.2 | 6.89 | |
| Yeast extract | 0.02 | g | 0.02 | - | |
| Distilled water | 1000.00 | ml | - | - | |
|
Agar
optional |
15.00 | g/l | - | - | |
|
D-Glucose*
(optional) |
1.00 | g/l | - | - | |
|
Cellobiose*
(optional) |
1.00 | g/l | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 Place a strip of sterile cellulose filter paper onto an agar plate. Alternatively, place a strip of sterile cellulose filter paper into a culture tube containing 5 ml of medium, thereby leaving 1 - 2 cm of the strip outside of the medium. Inoculate the strain onto the cellulose filter paper. The strain will grow on the cellulose paper only. | |||||
Metadata
| Last modified: | 19.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaNO3 | 2 | 23.531 |
| K2HPO4 | 1.2 | 6.89 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| KCl | 0.5 | 6.707 |
| KH2PO4 | 0.14 | 1.029 |
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