# 167: SPOROCYTOPHAGA MEDIUM


## Main sol. 167

2	g	NaNO3	
1	g	MgSO4 x 7 H2O	
0.5	g	KCl	
6	mg	FeSO4 x 7 H2O	
0.14	g	KH2PO4	
1.2	g	K2HPO4	
0.02	g	Yeast extract	
1000	ml	Distilled water	
15	g/l	Agar (optional)	

1. Adjust pH to 7.2.
2. Place a strip of sterile cellulose filter paper onto an agar plate. Alternatively, place a strip of sterile cellulose filter paper into a culture tube containing 5 ml of medium, thereby leaving 1 - 2 cm of the strip outside of the medium. Inoculate the strain onto the cellulose filter paper. The strain will grow on the cellulose paper only.