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HYPHOMICROBIUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Hyphomicrobium facile DSM 1565
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 162 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.36 | g | 1.36 | 9.994 | |
| Na2HPO4 | 2.13 | g | 2.13 | 15.004 | |
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 6.00 | mg | 0.006 | 0.041 | |
| FeSO4 x 7 H2O | 3.00 | mg | 0.003 | 0.011 | |
| MnSO4 x H2O | 1.00 | mg | 1.0e-3 | 0.006 | |
| Na2MoO4 x 2 H2O | 1.50 | mg | 0.002 | 0.006 | |
| Methylamine hydrochloride | 3.38 | g | 3.38 | 50.059 | |
| Yeast extract | 0.10 | g | 0.1 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.4 with NaOH. Autoclave 20 min at 121°C; final pH: 7.2. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 7 |
| Strains with modifications: | 1 |
Hyphomicrobium facile DSM 1565
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Methylamine hydrochloride | 3.38 | 50.059 |
| Na2HPO4 | 2.13 | 15.004 |
| KH2PO4 | 1.36 | 9.994 |
| (NH4)2SO4 | 0.5 | 3.784 |
| MgSO4 x 7 H2O | 0.2 | 0.811 |
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