Name: HYPHOMICROBIUM MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 1355 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
K₂HPO₄	2.50	g	2.5	14.353
NaH₂PO₄	2.25	g	2.25	18.754
(NH₄)₂SO₄	0.50	g	0.5	3.784
MgSO₄ x 7 H₂O	0.20	g	0.2	0.811
Visniac Trace Elements (1000x) , pH 6	1.00	ml	-	-
Distilled water	1000.00	ml	-	-
Agar , for solid medium	12.00	g	12	35.679
Methylamine (0.2% v/v)	2.00	ml	-	-
1 Adjust to pH 6 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. After autoclaving add sterile methylamine (0.2% v/v) to the sterile main solution.

Visniac Trace Elements (1000x) #

500

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	5.000	g	10	34.219
ZnSO₄ x 7 H₂O	2.200	g	4.4	15.301
CaCl₂ x 2 H₂O	0.733	g	1.466	9.972
MnCl₂ x 4 H₂O	0.506	g	1.012	5.114
FeSO₄ x 7 H₂O	0.499	g	0.998	3.59
(NH₄)₆Mo₇O₂₄ x 4 H₂O	0.110	g	0.22	-
CuSO₄ x 5 H₂O	0.157	g	0.314	1.258
CoCl₂ x 6 H₂O	0.161	g	0.322	2.48
Distilled water	500.000	ml	-	-
1 Adjust pH to 6.0 with NaOH.

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