Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.36 | g | 1.36 | 9.994 | |
| Na2HPO4 | 2.13 | g | 2.13 | 15.004 | |
| (NH4)2SO4 | 0.50 | g | 0.5 | 3.784 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 6.00 | mg | 0.006 | 0.041 | |
| FeSO4 x 7 H2O | 3.00 | mg | 0.003 | 0.011 | |
| MnSO4 x H2O | 1.00 | mg | 1.0e-3 | 0.006 | |
| Na2MoO4 x 2 H2O | 1.50 | mg | 0.002 | 0.006 | |
| Methylamine hydrochloride | 3.38 | g | 3.38 | 50.059 | |
| Yeast extract | 0.10 | g | 0.1 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.4 with NaOH. Autoclave 20 min at 121°C; final pH: 7.2. | |||||
Media with this solution
| 162 | HYPHOMICROBIUM MEDIUM |