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DSMZ

DSMZ 1542 . CY/H LIQUID MEDIUM FOR MYXOBACTERIA

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Kofleria flava DSM 53744

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 1542 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 1000 ml - -
Solution B 1 ml - -
Solution C 1 ml - -
1 Adjust pH of solution A to pH 7.4. Autoclave solutions A and B separately. Filter sterilize vitamin solution. After cooling, add 1 ml solution B and 1 ml solution C per liter solution A.

Solution A #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casitone 1.5 g 1.5 -
Yeast extract 1.5 g 1.5 -
Starch 4.0 g 4 -
Soy flour , defatted 1.0 g 1 -
D-Glucose 1.0 g 1 5.551
CaCl2 x 2 H2O 1.0 g 1 6.802
MgSO4 x 7 H2O 0.5 g 0.5 2.029
HEPES 11.9 g 11.9 49.937
Distilled water 1000.0 ml - -

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III)-EDTA 0.8 g 8 23.252
Distilled water 100.0 ml - -

Solution C #

100
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.05 g 0.5 0.369
Distilled water 100.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2
Kofleria flava DSM 53744

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
HEPES 11.9 49.937
Starch 4 -
Yeast extract 1.5 -
Casitone 1.5 -
CaCl2 x 2 H2O 1 6.802
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
phyla 1 3 strains
temperature mesophilic 3 strains