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DSMZ

DSMZ 1497 . HALOARCHAEUM SALINUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Halarchaeum salinum DSM 27207

This strain uses the standard medium without modifications.

Growth conditions: 37 °C

Main sol. 1497 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral Salts Solution for solid Medium , alternative 500 ml - -
Mineral Salts Solution for liquid Medium , alternative 500 ml - -

Mineral Salts Solution for solid Medium #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Glutamic acid 1.0 g 2 13.593
Trisodium citrate x 2 H2O 1.0 g 2 6.8
KH2PO4 0.5 g 1 7.348
NH4Cl 0.5 g 1 18.695
K2SO4 2.5 g 5 28.693
MgCl2 x 7 H2O 20.0 g 40 420.122
Casamino acids 2.0 g 4 -
Yeast extract
(Oxoid) 		1.0 g 2 -
FeSO4 2.0 mg 0.004 0.026
Trace Element Solution 1.0 ml - -
Distilled water 300.0 ml - -
NaCl 100.0 g 200 3422.31
Agar 20.0 g 40 118.931
Distilled water 200.0 ml - -

Mineral Salts Solution for liquid Medium #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Glutamic acid 1.0 g 2 13.593
Trisodium citrate x 2 H2O 1.0 g 2 6.8
KH2PO4 0.5 g 1 7.348
NH4Cl 0.5 g 1 18.695
K2SO4 2.5 g 5 28.693
MgCl2 x 7 H2O 20.0 g 40 420.122
NaCl 100.0 g 200 3422.31
Casamino acids 2.0 g 4 -
Yeast extract 1.0 g 2 -
FeSO4 2.0 mg 0.004 0.026
Trace Element Solution 1.0 ml - -
Distilled water 500.0 ml - -
1 Adjust to pH 5.5 with 10% KOH and sterilize by autoclaving.

Trace Element Solution #

1000
Solution occurs in 1 other media (first defined in Medium 1396).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CoCl2 x 6 H2O 0.30 g 0.3 2.311
MnCl2 x 4 H2O 0.10 g 0.1 0.505
ZnCl2 0.10 g 0.1 0.734
H3BO4 0.02 g 0.02 -
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
NiCl2 x 6 H2O 0.10 g 0.1 0.772
CuCl2 x 6 H2O 0.01 g 0.01 0.074
AlCl3 0.04 g 0.04 0.3
Na2WO4 x 2 H2O 0.02 g 0.02 0.061
CaCl2 x 2 H2O 1.00 g 1 6.802
BaCl2 x 2 H2O 0.01 g 0.01 0.041
Na2S2O3 x 5 H2O 2.00 g 2 8.058
Distilled water 1000.00 ml - -
1 Adjust to pH 4.0.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.5
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Associated strains

All strains for this medium: 1
Halarchaeum salinum DSM 27207

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 200 3422.31
MgCl2 x 7 H2O 40 420.122
Agar 40 118.931
K2SO4 5 28.693
Casamino acids 4 -
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
temperature mesophilic 1 strain
temperature thermophilic 1 strain