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DSMZ

DSMZ 1495 . AQUASPIRILLUM MEDIUM II

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Aquaspirillum bipunctata DSM 12821

This strain uses the standard medium without modifications.

Main sol. 1495 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone
(BD) 		2.00 g 2 -
Succinic acid 1.00 g 1 8.468
(NH4)2SO4 1.00 g 1 7.568
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 0.03 g 0.03 0.204
Vitamin solution 1.00 ml - -
Trace elements solution (Pfennig & Lippert, 1966) 1.00 ml - -
Distilled water 1000.00 ml - -
Vitamin solution 1.00 ml - -
Agar , for solid medium 15.00 g 15 44.599
1 Adjust pH to 7.4-7.6 Autoclave and add 1.0 ml/l vitamin solution from a filter sterilised stock solution (see below). The medium may be solidified by adding 15.0 g/l agar.

Trace elements solution (Pfennig & Lippert, 1966) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 6 H2O 2.20 g 2.2 14.482
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 1495).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 200 mg 0.2 0.819
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 100 mg 0.1 0.266
Nicotinic acid 50 mg 0.05 0.406
DL-Pantothenic acid 50 mg 0.05 0.228
Vitamin B12 1 mg 1.0e-3 7.4e-4
Para-aminobenzoic acid 50 mg 0.05 0.365
Distilled water 1000 ml - -
1 Filter sterilise.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.4 - 7.6
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 5
Aquaspirillum bipunctata DSM 12821

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Peptone 2 -
Succinic acid 1 8.468
(NH4)2SO4 1 7.568
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 5 strains BacDive

pH 6 - 8 2 strains
pH 8 - 10 2 strains
phyla 1 5 strains
oxygen aerobe 2 strains
oxygen facultative anaerobe 1 strain
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