Main sol. 1495
Internal Solution-ID: S3086
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Peptone
(BD) |
2.00 | g | 2 | - | |
| Succinic acid | 1.00 | g | 1 | 8.468 | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.204 | |
| Vitamin solution | 1.00 | ml | - | - | |
| Trace elements solution (Pfennig & Lippert, 1966) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| 1 Adjust pH to 7.4-7.6 Autoclave and add 1.0 ml/l vitamin solution from a filter sterilised stock solution (see below). The medium may be solidified by adding 15.0 g/l agar. | |||||
Media with this solution
| 1495 | AQUASPIRILLUM MEDIUM II |