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DSMZ

DSMZ 1485 . DESULFONATRONUM ALKALITOLERANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1485 #

ml
Equipment needed: Hungate tubes Gassing station Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 950 ml - -
Solution B 1 ml - -
Solution C 1 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
Solution G 10 ml - -
Solution H 10 ml - -
1 Dissolve ingredients of solution A except carbonates, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add bicarbonate and carbonate, adjust pH to 9.0, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions B to H prepared under 100% N2 gas atmosphere. Adjust pH of the complete medium to 8.7 - 9.0, if necessary.

Solution A #

950
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 6.0 g 6.316 108.073
K2HPO4 1.0 g 1.053 6.043
NaHCO3 32.0 g 33.684 400.969
Na2CO3 10.6 g 11.158 105.275
Na2SO4 2.8 g 2.947 20.75
Na-acetate 0.2 g 0.211 2.566
Distilled water 950.0 ml - -

Solution B #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace elements solution (Pfennig, 1965) 1 ml - -

Solution C #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Selenite-tungstate solution 1 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.2 g 20 373.902
Distilled water 10.0 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 0.2 g 20 98.375
Distilled water 10.0 ml - -

Solution F #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 20 mg 2 -
Distilled water 10 ml - -

Solution G #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-formate 3.4 g 340 4999.47
Distilled water 10.0 ml - -

Solution H #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -

Trace elements solution (Pfennig, 1965) #

1000
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 18.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.7 - 9.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 31.936 380.16
Na2CO3 10.579 99.812
NaCl 5.988 102.464
Na-formate 3.393 49.895
Na2SO4 2.794 19.673
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain