1464
1464 .
CHITINIVIBRIO MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Chitinivibrio alkaliphilus DSM 24538
* Modification: Use chitin C7170 by Sigma, others may not work as well
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: anaerobic
30 °C
Main sol. 1464 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaHCO3 | 15.0 | g/l | 15 | 178.557 | |
| Na2CO3 | 95.0 | g/l | 95 | 896.328 | |
| NaCl | 6.0 | g/l | 6 | 102.669 | |
| K2HPO4 | 1.0 | g/l | 1 | 5.741 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 10.0 with NaOH or HCl. Make anoxic under nitrogen stream and autoclave at 121°C. | |||||
| 2 After autoclaving add sterile, anoxic solutions of: | |||||
|
Chitin
, adjust volume in range 1-2 if required
(amorphous or Sigma C7170, 5% solution) |
1.5 | g | 1.5 | - | |
| MgSO4 | 1.0 | mM | 0.12 | 1 | |
| Yeast extract | 20.0 | mg | 0.02 | - | |
| NH4Cl | 4.0 | mM | 0.214 | 4 | |
| Na2S | 1.0 | mM | 0.079 | 1 | |
| Vitamine mixture (Pfennig & Lippert, 1965) | 1.0 | ml | - | - | |
| Acidic trace metals (Pfennig & Lippert, 1965) | 1.0 | ml | - | - | |
Solution occurs in 2
other media (first defined in Medium 1464).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Basic solution (100 ml 0.1 N NaOH) | 50 | ml | - | - | |
| Neutral solution (100 ml Aqua dem.) | 50 | ml | - | - | |
| Acidic solution (HCl, pH 3; 100 ml) | 50 | ml | - | - | |
| 1 Sterilize by filtration and keep at 4°C. Before addition to the medium mix 1:1:1 (vol:vol:vol). | |||||
Solution occurs in 2
other media (first defined in Medium 1464).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2 | mg | 0.02 | 0.082 |
| PABA | 5 | mg | 0.05 | 0.365 |
| Nicotinic acid | 5 | mg | 0.05 | 0.406 |
| Pyridoxine | 10 | mg | 0.1 | 0.591 |
| Distilled water | 100 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium 1464).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Folic acid | 2 | mg | 0.02 | 0.045 |
| Riboflavin | 5 | mg | 0.05 | 0.133 |
| Distilled water | 100 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium 1464).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5 | g/l | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2 | g/l | 2 | 7.194 | |
| ZnSO4 x 7 H2O | 100 | mg/l | 0.1 | 0.348 | |
| MnCl2 | 30 | mg/l | 0.03 | 0.238 | |
| H3BO3 | 300 | mg/l | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 200 | mg/l | 0.2 | 1.54 | |
| CuCl2 | 10 | mg/l | 0.01 | 0.074 | |
| NiCl2 x 2 H2O | 20 | mg/l | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 20 | mg/l | 0.02 | 0.083 | |
| Distilled water | 1000 | ml | - | - | |
| 1 adjust pH to 3-4 with HCl; autoclave in closed bottles, 120°C, 20 min. | |||||
Solution occurs in 2
other media (first defined in Medium 1464).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Thiamin | 5.0 | mg | 0.05 | 0.188 |
| Calcium pantothenate | 5.0 | mg | 0.05 | 0.105 |
| B12 | 0.5 | mg | 0.005 | 0.004 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 10.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 95 | 896.328 |
| NaHCO3 | 15 | 178.557 |
| NaCl | 6 | 102.669 |
| Chitin | 1.5 | - |
| K2HPO4 | 1 | 5.741 |
| Show more | ||
