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DSMZ

DSMZ 1464 . CHITINIVIBRIO MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1464 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 15.0 g/l 15 178.557
Na2CO3 95.0 g/l 95 896.328
NaCl 6.0 g/l 6 102.669
K2HPO4 1.0 g/l 1 5.741
Distilled water 1000.0 ml - -
1 Adjust pH to 10.0 with NaOH or HCl. Make anoxic under nitrogen stream and autoclave at 121°C.
2 After autoclaving add sterile, anoxic solutions of:
Chitin , adjust volume in range 1-2 if required
(amorphous or Sigma C7170, 5% solution) 		1.5 g 1.5 -
MgSO4 1.0 mM 0.12 1
Yeast extract 20.0 mg 0.02 -
NH4Cl 4.0 mM 0.214 4
Na2S 1.0 mM 0.079 1
Vitamine mixture (Pfennig & Lippert, 1965) 1.0 ml - -
Acidic trace metals (Pfennig & Lippert, 1965) 1.0 ml - -

Vitamine mixture (Pfennig & Lippert, 1965) #

150
Solution occurs in 2 other media (first defined in Medium 1464).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basic solution (100 ml 0.1 N NaOH) 50 ml - -
Neutral solution (100 ml Aqua dem.) 50 ml - -
Acidic solution (HCl, pH 3; 100 ml) 50 ml - -
1 Sterilize by filtration and keep at 4°C. Before addition to the medium mix 1:1:1 (vol:vol:vol).

Basic solution (100 ml 0.1 N NaOH) #

100
Solution occurs in 2 other media (first defined in Medium 1464).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2 mg 0.02 0.082
PABA 5 mg 0.05 0.365
Nicotinic acid 5 mg 0.05 0.406
Pyridoxine 10 mg 0.1 0.591
Distilled water 100 ml - -

Neutral solution (100 ml Aqua dem.) #

100
Solution occurs in 2 other media (first defined in Medium 1464).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Folic acid 2 mg 0.02 0.045
Riboflavin 5 mg 0.05 0.133
Distilled water 100 ml - -

Acidic trace metals (Pfennig & Lippert, 1965) #

1000
Solution occurs in 2 other media (first defined in Medium 1464).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 g/l 5 17.109
FeSO4 x 7 H2O 2 g/l 2 7.194
ZnSO4 x 7 H2O 100 mg/l 0.1 0.348
MnCl2 30 mg/l 0.03 0.238
H3BO3 300 mg/l 0.3 4.851
CoCl2 x 6 H2O 200 mg/l 0.2 1.54
CuCl2 10 mg/l 0.01 0.074
NiCl2 x 2 H2O 20 mg/l 0.02 0.154
Na2MoO4 x 2 H2O 20 mg/l 0.02 0.083
Distilled water 1000 ml - -
1 adjust pH to 3-4 with HCl; autoclave in closed bottles, 120°C, 20 min.

Acidic solution (HCl, pH 3; 100 ml) #

100
Solution occurs in 2 other media (first defined in Medium 1464).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamin 5.0 mg 0.05 0.188
Calcium pantothenate 5.0 mg 0.05 0.105
B12 0.5 mg 0.005 0.004
Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 10.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 95 896.328
NaHCO3 15 178.557
NaCl 6 102.669
Chitin 1.5 -
K2HPO4 1 5.741
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains