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Acidic trace metals (Pfennig & Lippert, 1965)

Internal Solution-ID: S5704

This solution is used in 3 media and was first defined in medium 1464.

Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 g/l 5 17.109
FeSO4 x 7 H2O 2 g/l 2 7.194
ZnSO4 x 7 H2O 100 mg/l 0.1 0.348
MnCl2 30 mg/l 0.03 0.238
H3BO3 300 mg/l 0.3 4.851
CoCl2 x 6 H2O 200 mg/l 0.2 1.54
CuCl2 10 mg/l 0.01 0.074
NiCl2 x 2 H2O 20 mg/l 0.02 0.154
Na2MoO4 x 2 H2O 20 mg/l 0.02 0.083
Distilled water 1000 ml - -
1 adjust pH to 3-4 with HCl; autoclave in closed bottles, 120°C, 20 min.
1464 CHITINIVIBRIO MEDIUM
1465 CHITINISPIRILLUM MEDIUM
1569 ORENIA CHITINITROPHA