Acidic trace metals (Pfennig & Lippert, 1965)
Internal Solution-ID: S5704
This solution is used in 3 media and was first defined in medium 1464.
Internal Solution-ID: S5704
This solution is used in 3 media and was first defined in medium 1464.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
EDTA | 5 | g/l | 5 | 17.109 | |
FeSO4 x 7 H2O | 2 | g/l | 2 | 7.194 | |
ZnSO4 x 7 H2O | 100 | mg/l | 0.1 | 0.348 | |
MnCl2 | 30 | mg/l | 0.03 | 0.238 | |
H3BO3 | 300 | mg/l | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 200 | mg/l | 0.2 | 1.54 | |
CuCl2 | 10 | mg/l | 0.01 | 0.074 | |
NiCl2 x 2 H2O | 20 | mg/l | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 20 | mg/l | 0.02 | 0.083 | |
Distilled water | 1000 | ml | - | - | |
1 adjust pH to 3-4 with HCl; autoclave in closed bottles, 120°C, 20 min. |