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DSMZ

DSMZ 144 . THERMOANAEROBACTER BROCKII MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Thermoanaerobacter thermohydrosulfuricus DSM 15750

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 65 °C

Main sol. 144 #

ml
Solution occurs in 1 other media (first defined in Medium 144).
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.90 g 0.9 16.826
NaCl 0.90 g 0.9 15.4
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KH2PO4 0.75 g 0.75 5.511
K2HPO4 1.50 g 1.5 8.612
Trace element solution 9.00 ml - -
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		3.00 ml 0.003 0.011
Yeast extract 3.00 g 3 -
Trypticase peptone
(BD BBL) 		10.00 g 10 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Wolin's vitamin solution 5.00 ml - -
D-Glucose 5.00 g 5 27.753
Na2S x 9 H2O 1.00 g 1 4.164
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except vitamins, glucose and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.2 - 7.4.

Trace element solution #

1000
Solution occurs in 8 other media (first defined in Medium 144).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		12.80 g 12.8 66.967
FeCl2 x 4 H2O 0.20 g 0.2 1.006
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.17 g 0.17 1.309
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.

Wolin's vitamin solution #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 06.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 16
Strains with modifications: 2
Thermoanaerobacter thermohydrosulfuricus DSM 15750

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase peptone 10 -
D-Glucose 5 27.753
Yeast extract 3 -
K2HPO4 1.5 8.612
Na2S x 9 H2O 1 4.164
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Cultivation metadata from 16 strains BacDive

pH 6 - 8 3 strains
pH 4 - 6 2 strains
phyla 3 17 strains
nutrition chemoorganotroph 1 strain
oxygen anaerobe 16 strains
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