Main sol. 144
Internal Solution-ID: S249
This solution is used in 2 media and was first defined in medium 144.
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Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.90 | g | 0.9 | 16.826 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| KH2PO4 | 0.75 | g | 0.75 | 5.511 | |
| K2HPO4 | 1.50 | g | 1.5 | 8.612 | |
| Trace element solution | 9.00 | ml | - | - | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
3.00 | ml | 0.003 | 0.011 | |
| Yeast extract | 3.00 | g | 3 | - | |
|
Trypticase peptone
(BD BBL) |
10.00 | g | 10 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Wolin's vitamin solution | 5.00 | ml | - | - | |
| D-Glucose | 5.00 | g | 5 | 27.753 | |
| Na2S x 9 H2O | 1.00 | g | 1 | 4.164 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except vitamins, glucose and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.2 - 7.4. | |||||