Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.34 | g | 0.336 | 4.502 | |
MgCl2 x 6 H2O | 4.00 | g | 3.949 | 19.423 | |
MgSO4 x 7 H2O | 3.45 | g | 3.406 | 13.818 | |
NH4Cl | 0.25 | g | 0.247 | 4.614 | |
CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.94 | |
K2HPO4 | 0.14 | g | 0.138 | 0.793 | |
NaCl | 18.00 | g | 17.769 | 304.055 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Fe(NH4)2(SO4)2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | - | |
Na-acetate | 1.00 | g | 0.987 | 12.034 | |
Yeast extract
(OXOID) |
2.00 | g | 1.974 | - | |
Trypticase peptone
(BD BBL) |
2.00 | g | 1.974 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
Wolin's vitamin solution | 10.00 | ml | - | - | |
NaHCO3 | 5.00 | g | 4.936 | 58.755 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.494 | 2.81 | |
Na2S x 9 H2O | 0.50 | g | 0.494 | 2.055 | |
Distilled water | 1000.00 | ml | - | - | |
1 Note: If the medium is being used without overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. | |||||
2 Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate and adjust pH to 6.5, then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 6.8 - 7.0, if necessary. | |||||
3 For incubation use sterile 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x H2O | 0.50 | g | 0.5 | 2.958 | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.18 | g | 0.18 | 1.161 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.18 | g | 0.18 | 0.626 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 x 12 H2O | 0.02 | g | 0.02 | 0.042 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 | |
Na2WO4 x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 21.02.24 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.8 - 7.0 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 34 |
Strains with modifications: | 14 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 17.769 | 304.055 |
NaHCO3 | 4.936 | 58.755 |
MgCl2 x 6 H2O | 3.949 | 19.423 |
MgSO4 x 7 H2O | 3.406 | 13.818 |
Trypticase peptone | 1.974 | - |
Show more |
pH | 6 - 8 | 6 strains |
pH | 4 - 6 | 5 strains |
pH | 8 - 10 | 4 strains |
phyla | 2 | 33 strains |
oxygen | anaerobe | 32 strains |
Show more |