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DSMZ

Strain modifications for Medium 141

9 distinct modifications were found.

Add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 80.00 mg/l.

Modified strains

Methanococcus voltae DSM 4254

Annotated modifications

0.08 g L-Histidine

Adjust pH to 6.5.

Modified strains

Methanoculleus marisnigri DSM 1498
Methanobacterium petrolearium DSM 22353

Annotated modifications

6.5

Adjust pH to 7.5 - 8.0.

Modified strains

Methanobacterium kanagiense DSM 22026

Annotated modifications

7.5 - 8.0

Adjust pH to 7.5.

Modified strains

Methanobacterium aarhusense DSM 15219
Methanoculleus receptaculi DSM 18860
Methanoeremita antiquus DSM 21220

Annotated modifications

7.5

Increase the amount of trypticase to 6.00 g/l.

Modified strains

Methanoculleus thermophilus DSM 2373

Annotated modifications

Trypticase peptone
6.00 g Trypticase peptone

Supplement medium after autoclaving with 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under 100% N2 gas. Adjust pH to 6.5 and use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Modified strains

Methanogenium marinum DSM 15558

Annotated modifications

6.5
0.50 g 2-Mercaptoethanesulfonic acid

Supplement medium after autoclaving with 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under 100% N2 gas. Adjust pH to 7.5 and use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Modified strains

Methanogenium frigidum DSM 16458

Annotated modifications

7.5
0.50 g 2-Mercaptoethanesulfonic acid

Supplement medium with 10 ml of a methanol solution (50% v/v) added to the autoclaved medium from a sterile anoxic stock solution.

Modified strains

Methanosphaera cuniculi DSM 4103

Annotated modifications

5 ml Methanol

Use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Modified strains

Methanothermobacter thermoflexus DSM 7268
Methanothermobacter defluvii DSM 7466
Methanococcus maripaludis DSM 14266
Modifications
Add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 80.00 mg/l. Adjust pH to 6.5. Adjust pH to 7.5 - 8.0. Adjust pH to 7.5. Increase the amount of trypticase to 6.00 g/l. Supplement medium after autoclaving with 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under 100% N2 gas. Adjust pH to 6.5 and use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture. Supplement medium after autoclaving with 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under 100% N2 gas. Adjust pH to 7.5 and use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture. Supplement medium with 10 ml of a methanol solution (50% v/v) added to the autoclaved medium from a sterile anoxic stock solution. Use only one atmosphere overpressure of sterile 80% H2 and 20% CO2 gas mixture.