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DSMZ

DSMZ 1396 . HALOARCHAEAL MEDIUM MH-1

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Halarchaeum acidiphilum DSM 22442

* Modification: The MgCl2 x 6H2O concentration of strain MH1-52-1 (DSM 22442) for well growth is 10.0 mM (20.0 g/l).

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 37 °C

Main sol. 1396 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Glutamic acid 2 g 2.494 16.949
Trisodium citrate x 2 H2O 2 g 2.494 8.479
KH2PO4 1 g 1.247 9.163
NH4Cl 1 g 1.247 23.311
K2SO4 5 g 6.234 35.777
NaCl 200 g 249.377 4267.22
Casamino acids 4 g 4.988 -
Yeast extract 2 g 2.494 -
FeSO4 x 6 H2O 4 mg 0.005 0.033
Trace Element Solution 2 ml - -
Agar 20 g 24.938 74.146
MgCl2 x 6 H2O* 20 g/l - -
Distilled water 800 ml - -
1 Adjust to pH 4.5 with 40% KOH and sterilize by autoclaving.

Trace Element Solution #

1000
Solution occurs in 1 other media (first defined in Medium 1396).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CoCl2 x 6 H2O 0.30 g 0.3 2.311
MnCl2 x 4 H2O 0.10 g 0.1 0.505
ZnCl2 0.10 g 0.1 0.734
H3BO4 0.02 g 0.02 -
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
NiCl2 x 6 H2O 0.10 g 0.1 0.772
CuCl2 x 6 H2O 0.01 g 0.01 0.074
AlCl3 0.04 g 0.04 0.3
Na2WO4 x 2 H2O 0.02 g 0.02 0.061
CaCl2 x 2 H2O 1.00 g 1 6.802
BaCl2 x 2 H2O 0.01 g 0.01 0.041
Na2S2O3 x 5 H2O 2.00 g 2 8.058
Distilled water 1000.00 ml - -
1 Adjust to pH 4.0.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 4.5
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Associated strains

All strains for this medium: 2
Strains with modifications: 2
Halarchaeum acidiphilum DSM 22442

  20 g/l MgCl2 x 6 H2O

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 249.377 4267.22
Agar 24.938 74.146
K2SO4 6.234 35.777
Casamino acids 4.988 -
L-Glutamic acid 2.494 16.949
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 2 strains
pH 6 - 8 1 strain
phyla 1 2 strains
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
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