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DSMZ

DSMZ 1372 . SUCCINATE MINIMAL SALTS MEDIUM (SMS)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Rhodomicrobium vannielii DSM 23296

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days 28 °C

Main sol. 1372 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.010 g 0.01 0.034
KH2PO4 0.600 g 0.6 4.409
K2HPO4 0.900 g 0.9 5.167
NH4Cl 1.000 g 1 18.695
MgSO4 x 7 H2O 0.200 g 0.2 0.811
CaCl2 x 6 H2O 0.075 g 0.075 0.342
Sodium succinate 2.200 g 2.2 13.576
Yeast extract 0.100 g 0.1 -
Trace elements solution 2.000 ml - -
Vitamin solution 2.000 ml - -
Distilled water 1000.000 ml - -
1 Adjust pH to 6.8.
2 Prepare the medium without the vitamin solution.
3 Autoclave and add the vitamin solution from a filter-sterilised stock solution.
4 Incubate strains in liquid culture with air headspace in closed vessels.

Trace elements solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 6 H2O 0.3 g 0.3 1.975
ZnSO4 x 7 H2O 5.0 mg 0.005 0.017
MnCl2 x 4 H2O 3.0 mg 0.003 0.015
H3BO3 2.0 mg 0.002 0.032
CoCl2 x 6 H2O 5.0 mg 0.005 0.039
CuCl2 x 2 H2O 1.0 mg 1.0e-3 0.006
NiCl2 x 6 H2O 2.0 mg 0.002 0.015
Na2MoO4 x 2 H2O 3.0 mg 0.003 0.012
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 1072).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 80 mg 0.08 0.327
Thiamine-HCl x 2 H2O 400 mg 0.4 1.072
Nicotinic acid 400 mg 0.4 3.249
Vitamin B12 20 mg 0.02 0.015
Distilled water 1000 ml - -

Metadata

Last modified: 08.05.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 5
Rhodomicrobium vannielii DSM 23296

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium succinate 2.2 13.576
NH4Cl 1 18.695
K2HPO4 0.9 5.167
KH2PO4 0.6 4.409
MgSO4 x 7 H2O 0.2 0.811
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Cultivation metadata from 5 strains BacDive

phyla 1 5 strains
temperature mesophilic 5 strains