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Main sol. 1372

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Internal Solution-ID: S2768

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.010 g 0.01 0.034
KH2PO4 0.600 g 0.6 4.409
K2HPO4 0.900 g 0.9 5.167
NH4Cl 1.000 g 1 18.695
MgSO4 x 7 H2O 0.200 g 0.2 0.811
CaCl2 x 6 H2O 0.075 g 0.075 0.342
Sodium succinate 2.200 g 2.2 13.576
Yeast extract 0.100 g 0.1 -
Trace elements solution 2.000 ml - -
Vitamin solution 2.000 ml - -
Distilled water 1000.000 ml - -
1 Adjust pH to 6.8.
2 Prepare the medium without the vitamin solution.
3 Autoclave and add the vitamin solution from a filter-sterilised stock solution.
4 Incubate strains in liquid culture with air headspace in closed vessels.
1372 SUCCINATE MINIMAL SALTS MEDIUM (SMS)