MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1372 . SUCCINATE MINIMAL SALTS MEDIUM (SMS)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Rhodomicrobium vannielii DSM 23282

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days 28 °C

Main sol. 1372 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.010 g 0.01 0.034
KH2PO4 0.600 g 0.6 4.409
K2HPO4 0.900 g 0.9 5.167
NH4Cl 1.000 g 1 18.695
MgSO4 x 7 H2O 0.200 g 0.2 0.811
CaCl2 x 6 H2O 0.075 g 0.075 0.342
Sodium succinate 2.200 g 2.2 13.576
Yeast extract 0.100 g 0.1 -
Trace elements solution 2.000 ml - -
Vitamin solution 2.000 ml - -
Distilled water 1000.000 ml - -
1 Adjust pH to 6.8.
2 Prepare the medium without the vitamin solution.
3 Autoclave and add the vitamin solution from a filter-sterilised stock solution.
4 Incubate strains in liquid culture with air headspace in closed vessels.

Trace elements solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 6 H2O 0.3 g 0.3 1.975
ZnSO4 x 7 H2O 5.0 mg 0.005 0.017
MnCl2 x 4 H2O 3.0 mg 0.003 0.015
H3BO3 2.0 mg 0.002 0.032
CoCl2 x 6 H2O 5.0 mg 0.005 0.039
CuCl2 x 2 H2O 1.0 mg 1.0e-3 0.006
NiCl2 x 6 H2O 2.0 mg 0.002 0.015
Na2MoO4 x 2 H2O 3.0 mg 0.003 0.012
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 1072).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 80 mg 0.08 0.327
Thiamine-HCl x 2 H2O 400 mg 0.4 1.072
Nicotinic acid 400 mg 0.4 3.249
Vitamin B12 20 mg 0.02 0.015
Distilled water 1000 ml - -

Metadata

Last modified: 08.05.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 5
Rhodomicrobium vannielii DSM 23282

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium succinate 2.2 13.576
NH4Cl 1 18.695
K2HPO4 0.9 5.167
KH2PO4 0.6 4.409
MgSO4 x 7 H2O 0.2 0.811
Show more
CSV
JSON

Cultivation metadata from 5 strains BacDive

phyla 1 5 strains
temperature mesophilic 5 strains