MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 13 . BACILLUS ACIDOCALDARIUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 13 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
1 Sterilize solution A and B separately at 121°C for 15 min. Cool to 50°C and then combine.

Solution A #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 2 -
(NH4)2SO4 0.20 g 0.4 3.027
MgSO4 x 7 H2O 0.50 g 1 4.057
CaCl2 x 2 H2O 0.25 g 0.5 3.401
KH2PO4 0.60 g 1.2 8.818
Distilled water 500.00 ml - -
1 Adjust pH to 3.0 - 4.0.

Solution B #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 1 g 2 11.101
Agar 20 g 40 118.931
Distilled water 500 ml - -

Metadata

Last modified: 04.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 8
Strains with modifications: 7

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.466
Glucose 1 5.551
Yeast extract 1 -
KH2PO4 0.6 4.409
MgSO4 x 7 H2O 0.5 2.029
Show more
CSV
JSON

Cultivation metadata from 8 strains BacDive

pH 4 - 6 6 strains
phyla 1 8 strains
oxygen aerobe 6 strains
temperature thermophilic 8 strains