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DSMZ

DSMZ 1228 . THIOALKALIVIBRIO THIOCYANODENITRIFICANS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Thioalkalivibrio thiocyanodenitrificans DSM 16954

This strain uses the standard medium without modifications.

Growth conditions: 7-14 days aerobic 28 °C

Main sol. 1228 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 22 g 22 207.571
NaHCO3 8 g 8 95.23
NaCl 5 g 5 85.558
K2HPO4 1 g 1 5.741
Distilled water 1000 ml - -
1 Sterilize at 120°C 20 min in a closed vessel (i.e. a serum tube or bottle) with a liquid to air ratio of about 1:10. pH after sterilization will about 10.
2 After sterilization add from sterile stock solutions:
Trace element solution 2 ml - -
MgCl2 x 6 H2O
(200.0 g/l) 		1 ml - -
3 (a white colloid will form which will rapidly dissolve after mixing)
Sodium thiosulfate pentahydrate
(4 M - 992 g/l) 		10 ml 9.927 40
KSCN
(2 M - 194 g/l) 		5 ml 0.972 10
4 Incubate statically until growth occurs, then shake at 100 rpm.

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 925).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5 mg 0.005 0.017
FeSO4 x 7 H2O 2 mg 0.002 0.007
ZnSO4 x 7 H2O 100 mg 0.1 0.348
MnCl2 x 4 H2O 30 mg 0.03 0.152
CoCl2 x 6 H2O 200 mg 0.2 1.54
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 30 mg 0.03 0.124
CuCl2 x 2 H2O 10 mg 0.01 0.059
H3BO3 300 mg 0.3 4.851
Distilled water 1000 ml - -
1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 10.0
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Associated strains

All strains for this medium: 1
Thioalkalivibrio thiocyanodenitrificans DSM 16954

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 22 207.571
Sodium thiosulfate pentahydrate 9.927 40
NaHCO3 8 95.23
NaCl 5 85.558
K2HPO4 1 5.741
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain