| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 22 | g | 22 | 207.571 | |
| NaHCO3 | 8 | g | 8 | 95.23 | |
| NaCl | 5 | g | 5 | 85.558 | |
| K2HPO4 | 1 | g | 1 | 5.741 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Sterilize at 120°C 20 min in a closed vessel (i.e. a serum tube or bottle) with a liquid to air ratio of about 1:10. pH after sterilization will about 10. | |||||
| 2 After sterilization add from sterile stock solutions: | |||||
| Trace element solution | 2 | ml | - | - | |
|
MgCl2 x 6 H2O
(200.0 g/l) |
1 | ml | - | - | |
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
|
Sodium thiosulfate pentahydrate
(4 M - 992 g/l) |
10 | ml | 9.927 | 40 | |
|
KSCN
(2 M - 194 g/l) |
5 | ml | 0.972 | 10 | |
| 4 Incubate statically until growth occurs, then shake at 100 rpm. | |||||
Media with this solution
| 1228 | THIOALKALIVIBRIO THIOCYANODENITRIFICANS MEDIUM |