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DSMZ

DSMZ 1191 . EGGC

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Thiolinea disciformis DSM 14473

This strain uses the standard medium without modifications.

Growth conditions: 22 °C

Main sol. 1191 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-acetate 0.3 g 0.3 3.657
Casamino acids 0.3 g 0.3 -
Glucose 0.6 g 0.6 3.33
Salt solution 10.0 ml - -
Agar 15.0 g 15 44.599
Demineralized water 1000.0 ml - -
1 Please add after autoclaving:
Vitamin solution 5.0 ml - -
Phosphate solution 2.0 ml - -

Salt solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.0 g 1 7.568
KCl 0.5 g 0.5 6.707
MgSO4 x 7 H2O 0.5 g 0.5 2.029
CaCl2 x 2 H2O 0.2 g 0.2 1.36
CaCO3 0.2 g 0.2 1.998
FeCl3 x 6 H2O 5.0 mg 0.005 0.018
Demineralized water 1000.0 ml - -

Phosphate solution #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 2.480 g 124 873.492
NaH2PO4 0.308 g 15.4 128.358
Demineralized water 20.000 ml - -
1 Adjust pH to 7.2

Vitamin solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Riboflavin 10.0 mg 0.1 0.266
Thiamine-HCl x 2 H2O 50.0 mg 0.5 1.339
Nicotinic acid 50.0 mg 0.5 4.061
Pyridoxine hydrochloride 50.0 mg 0.5 2.431
Calcium pantothenate 50.0 mg 0.5 1.049
Biotin 0.1 mg 1.0e-3 0.004
Folic acid 0.2 mg 0.002 0.005
Vitamin B12 1.0 mg 0.01 0.007
Demineralized water 100.0 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 5
Strains with modifications: 2
Thiolinea disciformis DSM 14473

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Glucose 0.6 3.33
Na-acetate 0.3 3.657
Casamino acids 0.3 -
Na2HPO4 0.248 1.747
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Cultivation metadata from 5 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 5 strains
nutrition chemolithoautotroph 1 strain
temperature mesophilic 5 strains