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DSMZ

DSMZ 1131 . HYDROGENIVIRGA OKINAWENSIS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Thiolapillus brandeum DSM 23672

* Modification: apply 2 bar H2/CO2 (80:20)

Growth conditions: 2-3 days 30 °C

Main sol. 1131 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Modified MJ synthetic seawater 1000 ml - -
1 Sparge medium with 100% N2 gas mixture for 10 min to make anoxic.
2 Adjust pH to 7.0 with NaOH.
3 Sparge medium with 80% N2 and 20% CO2 gas mixture for 10 min.
Sulfur
(powder) 		3 g/l 3 93.545
4 Dispense the medium (10 mL) into 100 mL serum bottles, containing 0,03g sulfur (powder) under a stream of H2/CO2 (80:20) and seal with butyl rubber stoppers.
5 Autoclave at 121 °C for 20 min.
6 Prior to inoculation, add vitamins, NaNO3, and NaHCO3 (from filter sterilized stock solutions under a N2 gas atmosphere) to the medium.
Vitamin solution 10 ml/l - -
NaNO3 1 g/l 1 11.765
NaHCO3 1 g/l 1 11.904

Modified MJ synthetic seawater #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g - -
K2HPO4 0.14 g - -
CaCl2 x 2 H2O 0.50 g - -
NH4Cl 0.50 g - -
MgSO4 x 7 H2O 3.40 g - -
MgCl2 x 6 H2O 4.18 g - -
KCl 0.33 g - -
FeSO4 x 7 H2O 0.01 g - -
NiCl2 x 6 H2O 1.00 mg - -
Na2SeO3 x 5 H2O 1.00 mg - -
Modified Wolin's mineral solution II 10.00 ml - -
Distilled water 1000.00 ml - -

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Modified Wolin's mineral solution II #

1000
Solution occurs in 24 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 20.03.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Gas composition

  • 80 % H2
  • 20 % CO2
  • 2 % bar

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 2
Thiolapillus brandeum DSM 23672

  80% H2, 20% CO2, 2 bar

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 4.139 20.357
Sulfur 3 93.545
NaHCO3 1 11.904
NaNO3 1 11.765
NH4Cl 0.495 9.255
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 2 strains
oxygen aerobe 1 strain
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