Main sol. 1131
Internal Solution-ID: S2267
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified MJ synthetic seawater | 1000 | ml | - | - | |
| 1 Sparge medium with 100% N2 gas mixture for 10 min to make anoxic. | |||||
| 2 Adjust pH to 7.0 with NaOH. | |||||
| 3 Sparge medium with 80% N2 and 20% CO2 gas mixture for 10 min. | |||||
|
Sulfur
(powder) |
3 | g/l | 3 | 93.545 | |
| 4 Dispense the medium (10 mL) into 100 mL serum bottles, containing 0,03g sulfur (powder) under a stream of H2/CO2 (80:20) and seal with butyl rubber stoppers. | |||||
| 5 Autoclave at 121 °C for 20 min. | |||||
| 6 Prior to inoculation, add vitamins, NaNO3, and NaHCO3 (from filter sterilized stock solutions under a N2 gas atmosphere) to the medium. | |||||
| Vitamin solution | 10 | ml/l | - | - | |
| NaNO3 | 1 | g/l | 1 | 11.765 | |
| NaHCO3 | 1 | g/l | 1 | 11.904 | |
Media with this solution
| 1131 | HYDROGENIVIRGA OKINAWENSIS MEDIUM |