110
110 .
CHOPPED MEAT MEDIUM WITH CARBOHYDRATES
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Eubacterium nodatum DSM 3993
* Modification: Plus haemine (5µg/ml) plus Vit K1; anaerobic
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 1-2 days
anaerobic
37 °C
pH 7.00
Main sol. 110 #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Ground beef
(fat free) |
500.0 | g | 500 | - | |
| Distilled water | 1000.0 | ml | - | - | |
|
NaOH
(1 N) |
25.0 | ml | - | - | |
| 1 Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: | |||||
| Casitone | 30.0 | g | 30 | - | |
| Yeast extract | 5.0 | g | 5 | - | |
| K2HPO4 | 5.0 | g | 5 | 28.707 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| D-Glucose | 4.0 | g | 4 | 22.203 | |
| Cellobiose | 1.0 | g | 1 | 2.921 | |
| Maltose | 1.0 | g | 1 | 2.921 | |
|
Starch
(soluble) |
1.0 | g | 1 | - | |
| L-Cysteine HCl , add to make medium anoxic | 0.5 | g | 0.5 | 3.172 | |
| Agar , if required | 15.0 | g | 15 | 44.599 | |
| 2 In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: and either or | |||||
| Haemin solution | 10.0 | ml | - | - | |
| Vitamin K1 solution , alternative | 10.0 | ml | - | - | |
| Vitamin K3 solution | 10.0 | ml | - | - | |
| Haemin* | 5.0 | mg/l | - | - | |
| 3 To make medium anoxic boil it, cool under 100% N2 gas atmosphere, add 0.5 g/l L-cysteine hydrochloride and adjust pH to 7.0. Dispense under same gas atmosphere 7 ml medium into Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121°C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Haemin | 50 | mg | 0.5 | 0.767 | |
|
NaOH
(1 N) |
1 | ml | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin K1 | 0.1 | ml | - | - | |
|
Ethanol
(95 %) |
20.0 | ml | 950 | 20620.8 | |
| 1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin K3 | 5 | mg | 0.05 | - | |
|
Ethanol
(95%) |
1 | ml | 9.5 | 206.208 | |
| Water | 100 | ml | - | - | |
| 1 Dissolve 5 mg vitamin K3 in 1 ml 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Filter, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Ground beef | 500 | - |
| Casitone | 30 | - |
| Agar | 15 | 44.599 |
| K2HPO4 | 5 | 28.707 |
| Yeast extract | 5 | - |
| Show more | ||
Cultivation metadata from 246 strains
| halophily | non-halophilic | 2 strains |
| pH | 6 - 8 | 12 strains |
| pH | 8 - 10 | 6 strains |
| pH | 4 - 6 | 6 strains |
| phyla | 10 | 239 strains |
| Show more | ||