1076b
1076b .
SP4-Z MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Mycoplasma tullyi DSM 21909
* Modification: Plus glucose; plus 5% CO2
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 2-3 days
aerobic + 5% CO2
37 °C
pH 7.00
Main sol. 1076b #
ml
| 1 The difference between this medium and "normal" SP4 medium (Medium 1076) is that the yeast extract was left out and DNA was added instead. Since most mycoplasmas use either glucose or arginine as their energy source one is added to the media. Do not combine both in one medium, arginine can inhibit the growth of some glucose utilizers (unknown reason). | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| PPLO broth | 0.70 | g | 3.5 | - | |
| Tryptone | 2.00 | g | 10 | 140.687 | |
|
Bacto peptone
(BD) |
1.00 | g | 5 | - | |
| Distilled water | 130.00 | ml | - | - | |
|
DNA
(fish sperm; SERVA) |
0.04 | g | 0.2 | - | |
| 2 Adjust pH to 7.4; autoclave at 121°C for 15 minutes. | |||||
| 3 Add aseptically: | |||||
|
CMRL 1066
(10x concentrated; GIBCO) |
10.00 | ml | - | - | |
|
Yeast extract
(BD; 2%, autoclaved) |
20.00 | ml | 0.4 | - | |
|
Fetal bovine serum
(heat-inactivated) |
17.00 | ml | - | - | |
|
Swine serum
(heat-inactivated) |
17.00 | ml | - | - | |
| 4 As energy source add either: | |||||
|
Glucose
OR
L-Arginine x HCl
(50 % aqueous) |
2.00 | ml | 1 | 5.551 | |
| 5 For Ureaplasma add instead of Glucose or Arginine: | |||||
| Urea | 0.40 | g | 2 | 33.302 | |
| Agar , for solid medium | 2.00 | g | 10 | 29.733 | |
| 6 For agar plates use only 126 ml of distilled water and add 2 g of agar. Medium containing agar should be placed at 55°C before adding supplements. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.4 |
| Edit in Medium builder | |
Gas composition
- 5 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Tryptone | 10 | 140.687 |
| Agar | 10 | 29.733 |
| Bacto peptone | 5 | - |
| PPLO broth | 3.5 | - |
| Urea | 2 | 33.302 |
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Cultivation metadata from 140 strains
| pH | 6 - 8 | 2 strains |
| phyla | 4 | 143 strains |
| oxygen | microaerophile | 29 strains |
| oxygen | aerobe | 9 strains |
| oxygen | facultative anaerobe | 3 strains |
| Show more | ||