105
105 .
GLUCONOBACTER OXYDANS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Gluconobacter oxydans DSM 50049
This strain uses the standard medium without modifications.
Growth conditions: 26 °C
Main sol. 105 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 100 | g | 100 | 555.062 | |
| Yeast extract | 10 | g | 10 | - | |
| CaCO3 | 20 | g | 20 | 199.82 | |
| Distilled water | 1000 | ml | - | - | |
| Agar , for solid medium | 15 | g/l | 15 | 44.599 | |
| 1 Adjust pH to 6.8. | |||||
| 2 Autoclave at 121 °C for 15 min. | |||||
| 3 Note: 20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. In liquid culture the calcium carbonate also forms a sediment that clears as acid is produced. | |||||
Metadata
| Last modified: | 08.11.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 40 |
| Strains with modifications: | 3 |
Gluconobacter oxydans DSM 50049
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 100 | 555.062 |
| CaCO3 | 20 | 199.82 |
| Agar | 15 | 44.599 |
| Yeast extract | 10 | - |
| Distilled water | - | - |
Cultivation metadata from 40 strains
| pH | 2 - 4 | 2 strains |
| phyla | 1 | 41 strains |
| oxygen | obligate aerobe | 9 strains |
| oxygen | aerobe | 3 strains |
| temperature | mesophilic | 40 strains |