{"medium":{"id":105,"name":"GLUCONOBACTER OXYDANS MEDIUM","complex_medium":"yes","min_pH":6.8,"max_pH":6.8,"source":"DSMZ","link":"https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium105.pdf"},"solutions":[{"id":181,"name":"Main sol. 105","volume":1000,"recipe":[{"recipe No.":1,"compound":"Glucose","compound_id":5,"amount":100,"unit":"g","g_l":100,"mmol_l":555.062,"optional":"no"},{"recipe No.":2,"compound":"Yeast extract","compound_id":16,"amount":10,"unit":"g","g_l":10,"optional":"no"},{"recipe No.":3,"compound":"CaCO3","compound_id":13,"amount":20,"unit":"g","g_l":20,"mmol_l":199.82,"optional":"no"},{"recipe No.":4,"compound":"Distilled water","compound_id":4,"amount":1000,"unit":"ml","optional":"no"},{"recipe No.":5,"compound":"Agar","compound_id":3,"condition":"for solid medium","amount":15,"unit":"g/l","g_l":15,"mmol_l":44.5991,"optional":"no"}],"steps":[{"step":"Adjust pH to 6.8."},{"step":"Autoclave at 121 \u00b0C for 15 min."},{"step":"Note: 20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. In liquid culture the calcium carbonate also forms a sediment that clears as acid is produced."}],"equipment":{"5329":"Autoclave"},"main solution":"yes"}]}