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Culture medium recipe

Medium for Acetobacter aceti DSM 2002

This strain uses the standard medium without modifications.

Growth conditions: 2-3 days aerobic 25 °C
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 100 g 100 555.062
Yeast extract 10 g 10 -
CaCO3 20 g 20 199.82
Distilled water 1000 ml - -
Agar , for solid medium 15 g/l 15 44.599
1 Adjust pH to 6.8.
2 20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. In liquid culture the calcium carbonate also forms a sediment that clears as acid is produced.


Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
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Associated strains

All strains for this medium: 40
Strains with modifications: 3
Acetobacter aceti DSM 2002

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 100 555.062
CaCO3 20 199.82
Agar 15 44.599
Yeast extract 10 -
Distilled water - -

Cultivation metadata from 40 strains BacDive

pH 2 - 4 2 strains
phyla 1 40 strains
oxygen obligate aerobe 9 strains
oxygen aerobe 3 strains
temperature mesophilic 40 strains