104b
104b .
PY + X MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Anaerosphaera aminiphila DSM 21120
* Modification: Replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1.0 ml/l of a sterile anoxic seven vitamins solution (see medium 503).
Growth conditions: anaerobic
30 °C
Main sol. 104b #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD BBL) |
5.0 | g | 5 | - | |
|
Meat peptone
(pepsin-digested) |
5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Salt solution | 40.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| D-Glucose* | 5.0 | g | 5 | 27.753 | |
| Na glutamate* | 5.0 | g | - | - | |
| Seven vitamins solution* | 1.0 | ml | - | - | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and glucose) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose or any other substrate from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.8 - 7.0, if necessary. | |||||
Solution occurs in 9
other media (first defined in Medium 104).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 78
other media (first defined in Medium 503).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 100 | mg | 0.1 | 0.074 |
| p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
| D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
| Nicotinic acid | 200 | mg | 0.2 | 1.625 |
| Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
| Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
| Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 10 | - |
| Na glutamate | 5 | 29.566 |
| Trypticase peptone | 5 | - |
| Meat peptone | 5 | - |
| L-Cysteine HCl x H2O | 0.5 | 2.847 |
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Cultivation metadata from 129 strains
| pH | 6 - 8 | 25 strains |
| pH | 4 - 6 | 20 strains |
| pH | 8 - 10 | 17 strains |
| pH | 2 - 4 | 1 strain |
| phyla | 4 | 129 strains |
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