Strain modifications for Medium 104b

Adjust pH of complete medium to 7.5.

Adjust pH to 8.0 with a sterile anoxic stock solution of Na₂CO₃ (5% w/v) prepared under 80% N₂ and 20% CO₂ gas atmosphere.

Adjust pH to 8.5 with a sterile anoxic stock solution of Na₂CO₃ (5% w/v) prepared under 80% N₂ and 20% CO₂ gas atmosphere.

Omit glucose.

Plus 30 mM Lysine, and vitamin mixture as indicated in medium 141; anaerobic

Reduce amount of D-glucose to 2.0 g/l.

Replace glucose by fructose (5g/L); strictly anaerobic

Replace glucose with 1.0 g/l cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH to 6.5.

Replace glucose with 2.0 g/l Na-pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration.

Replace glucose with 5.0 g/l cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH to 7.4 using a sterile anoxic stock solution of Na₂CO₃ (5% w/v) prepared under 80% N₂ and 20% CO₂ gas atmosphere

Replace glucose with 5.0 g/l D-fructose added after autoclaving from an anoxic stock solution and adjust pH to 7.5 - 8.0.

Replace glucose with 5.0 g/l D-fructose added after autoclaving from an anoxic stock solution.

Replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1.0 ml/l of a sterile anoxic seven vitamins solution (see medium 503).

Replace glucose with 5.0 g/l xylan.

Replace glucose with 8.0 g/l Na₂-succinate added after autoclaving from an anoxic stock solution sterilized by filtration.

Supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose added after autoclaving from an anoxic stock solution. Adjust pH to 7.3 - 7.5.