Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase peptone | 5.0 | g | 5 | - | |
Peptone | 5.0 | g | 5 | - | |
Yeast extract | 10.0 | g | 10 | - | |
Beef extract | 5.0 | g | 5 | - | |
Glycine | 3.0 | g | - | - | |
K2HPO4 | 2.0 | g | 2 | 11.483 | |
Tween 80 | 1.0 | ml | - | - | |
Salt solution | 40.0 | ml | - | - | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
Vitamin K1 solution | 0.2 | ml | - | - | |
Haemin solution | 10.0 | ml | - | - | |
L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
Distilled water | 950.0 | ml | - | - | |
1 The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Haemin | 50 | mg | 0.5 | 0.767 | |
NaOH
(1 N) |
1 | ml | - | - | |
Distilled water | 100 | ml | - | - | |
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin K1 | 0.1 | ml | - | - | |
Ethanol
(95 %) |
20.0 | ml | 950 | 20620.8 | |
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
K2HPO4 | 1.00 | g | 1 | 5.741 |
KH2PO4 | 1.00 | g | 1 | 7.348 |
NaHCO3 | 10.00 | g | 10 | 119.038 |
NaCl | 2.00 | g | 2 | 34.223 |
Distilled water | 1000.00 | ml | - | - |
Last modified: | 17.02.22 |
Source: | DSMZ |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Description:
Medium for Parvimonas micra (synonym Peptococcus glycinophilus) |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .