104
104 .
PYG MEDIUM (modified)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Eubacterium nodatum DSM 3993
* Modification: Plus haemine (5µg/ml) plus Vit K1
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 2-3 days
anaerobic
37 °C
pH 7.00
Main sol. 104 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 5 | - | |
| Peptone | 5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Beef extract | 5.0 | g | 5 | - | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| K2HPO4 | 2.0 | g | 2 | 11.483 | |
| Tween 80 | 1.0 | ml | - | - | |
| Salt solution | 40.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Vitamin K1 solution | 0.2 | ml | - | - | |
| Haemin solution | 10.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | - | |
| Haemin* | 5.0 | mg/l | - | - | |
| Distilled water | 950.0 | ml | - | - | |
| 1 The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave. | |||||
Solution occurs in 9
other media (first defined in Medium 104).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Haemin | 50 | mg | 0.5 | 0.767 | |
|
NaOH
(1 N) |
1 | ml | - | - | |
| Distilled water | 100 | ml | - | - | |
| 1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin K1 | 0.1 | ml | - | - | |
|
Ethanol
(95 %) |
20.0 | ml | 950 | 20620.8 | |
| 1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 10 | - |
| Glucose | 5 | 27.753 |
| Peptone | 5 | - |
| Trypticase peptone | 5 | - |
| Beef extract | 5 | - |
| Show more | ||
Cultivation metadata from 484 strains
| halophily | non-halophilic | 1 strain |
| pH | 6 - 8 | 33 strains |
| pH | 4 - 6 | 14 strains |
| pH | 8 - 10 | 13 strains |
| phyla | 13 | 465 strains |
| Show more | ||