Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 5 | - | |
| Peptone | 5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Beef extract | 5.0 | g | 5 | - | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| K2HPO4 | 2.0 | g | 2 | 11.483 | |
| Tween 80 | 1.0 | ml | - | - | |
| Salt solution | 40.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Vitamin K1 solution | 0.2 | ml | - | - | |
| Haemin solution | 10.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | - | |
| Distilled water | 950.0 | ml | - | - | |
| 1 The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave. | |||||
Media with this solution
| 104 | PYG MEDIUM (modified) |